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Download Green Fluorescent Protein Volume 302 Methods in Enzymology Download Online

Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. U is a Eckhard Jankowsky. Carl Wu. Lester Packer. Richard R. Harry Gilbert. Enrique Cadenas. Saurabh Jha. Dane Wittrup. Zvi Kelman.

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Ron Vale. Jon Lorsch. Sergey Tetin. Tracy Handel.

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Popular Features. New Releases. Michael Conn. Description One of the most important recent discoveries in science is the green fluorescent protein, isolated from a bioluminescent jellyfish. The gene that codes for this protein is active in any type of cell, from microbes to humans, producing a "day-glo" dye visible as a bright green light in the microscope. The power of the gene is that by using recombinant DNA technology.

GFP can be spliced to any protein, essentially painting that protein green. When such a spliced protein is inside a cell. Further, time-lapse microscopy can be used to examine how that protein and the cellular components that it is part of move within the cell during the cell's life cycle. In contrast, in less than four years thereafter, over references are listed, a testament to the rapid growth of interest in this probe.

This volume documents many diverse uses for this interesting molecule in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in previously published sources. The techniques are described in a context that allows comparisons to other related methodologies-such comparisons are valuable to readers who must adapt existing procedures to new systems. Also, so far as possible, methodologies have been presented in a manner that stresses their general applicability and potential limitations.

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The volume provides a substantial and current overview of the extant methodology in the field and a view of its rapid development. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since , each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than volumes all of them still in print , the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Product details Format Hardback pages Dimensions Other books in this series. Add to basket. Cellulases: Volume Harry Gilbert. Reconstituting the Cytoskeleton: Volume Ron Vale. Chemokines: Volume Tracy Handel. Table of contents Monitoring of Physiological Processes: S. Kupzig, S. San Lee and G. Banting, Membrane Trafficking.

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Kaether and H. Zhao, T. Duong, C. Huang, S. Kain and X.

What is GFP?

Kain and J. Hanakam and G. Cronin and R. Localization of Molecules: M.